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nuclear stain counterstaining  (Vector Laboratories)


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    Vector Laboratories nuclear stain counterstaining
    Nuclear Stain Counterstaining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/methyl+green+nuclear+stain/pm40408439-96-16-22?v=Vector+Laboratories
    Average 96 stars, based on 475 article reviews
    nuclear stain counterstaining - by Bioz Stars, 2026-06
    96/100 stars

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    Fig. 1. Coexpression analysis of sFRP-1 and mWnt-8 in embryonic heart of embryo from 9.5 (A±D), 11.5 (E±G), 12.5 (H±L) and 17.5 (M±O). I and K are, respectively, the heart structure part of H and L. sFRP-1 and mWnt-8 transcripts were detected respectively with a digoxigenin-labelled sFRP-1 (A,E,H,I,M) or mWnt-8 antisense probe (C,G,K,L,O) and revealed by blue staining. Sections were then counterstained with a <t>nuclear</t> <t>green</t> <t>stain.</t> sFRP-1 protein expression was detected by immunohistochemistry labeling (brown color) (B,D,F,J,N). mWnt-8 transcript expression overlapped with sFRP-1 transcripts and protein expression in the heart. sFRP-1 and mWnt-8 were detected in the wall of the aortic sac (AS), of the sinus venosus (SV) and in the trabeculated wall of the common ventricular heart chamber (V), in the muscular part of the interventricular septum (is) and in the wall of the common atrial chamber atrium (A), but not in the endocardial cushions (ec). sFRP-1 and mWnt-8 are highly expressed in the myocardium (m) from 9.5 to 12.5 d.p.c. but their expression decreased to be undetectable at 17.5 d.p.c. in the heart. No expression was observed in the pericardium (p) or the endocardium (arrow). Magni®cations £ 40 (A±C), £ 10 (D± G), £ 4 (H±L), £ 5 (M±O).
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    Fig. 1. Coexpression analysis of sFRP-1 and mWnt-8 in embryonic heart of embryo from 9.5 (A±D), 11.5 (E±G), 12.5 (H±L) and 17.5 (M±O). I and K are, respectively, the heart structure part of H and L. sFRP-1 and mWnt-8 transcripts were detected respectively with a digoxigenin-labelled sFRP-1 (A,E,H,I,M) or mWnt-8 antisense probe (C,G,K,L,O) and revealed by blue staining. Sections were then counterstained with a nuclear green stain. sFRP-1 protein expression was detected by immunohistochemistry labeling (brown color) (B,D,F,J,N). mWnt-8 transcript expression overlapped with sFRP-1 transcripts and protein expression in the heart. sFRP-1 and mWnt-8 were detected in the wall of the aortic sac (AS), of the sinus venosus (SV) and in the trabeculated wall of the common ventricular heart chamber (V), in the muscular part of the interventricular septum (is) and in the wall of the common atrial chamber atrium (A), but not in the endocardial cushions (ec). sFRP-1 and mWnt-8 are highly expressed in the myocardium (m) from 9.5 to 12.5 d.p.c. but their expression decreased to be undetectable at 17.5 d.p.c. in the heart. No expression was observed in the pericardium (p) or the endocardium (arrow). Magni®cations £ 40 (A±C), £ 10 (D± G), £ 4 (H±L), £ 5 (M±O).

    Journal: Mechanisms of development

    Article Title: Expression pattern of mouse sFRP-1 and mWnt-8 gene during heart morphogenesis.

    doi: 10.1016/s0925-4773(99)00236-1

    Figure Lengend Snippet: Fig. 1. Coexpression analysis of sFRP-1 and mWnt-8 in embryonic heart of embryo from 9.5 (A±D), 11.5 (E±G), 12.5 (H±L) and 17.5 (M±O). I and K are, respectively, the heart structure part of H and L. sFRP-1 and mWnt-8 transcripts were detected respectively with a digoxigenin-labelled sFRP-1 (A,E,H,I,M) or mWnt-8 antisense probe (C,G,K,L,O) and revealed by blue staining. Sections were then counterstained with a nuclear green stain. sFRP-1 protein expression was detected by immunohistochemistry labeling (brown color) (B,D,F,J,N). mWnt-8 transcript expression overlapped with sFRP-1 transcripts and protein expression in the heart. sFRP-1 and mWnt-8 were detected in the wall of the aortic sac (AS), of the sinus venosus (SV) and in the trabeculated wall of the common ventricular heart chamber (V), in the muscular part of the interventricular septum (is) and in the wall of the common atrial chamber atrium (A), but not in the endocardial cushions (ec). sFRP-1 and mWnt-8 are highly expressed in the myocardium (m) from 9.5 to 12.5 d.p.c. but their expression decreased to be undetectable at 17.5 d.p.c. in the heart. No expression was observed in the pericardium (p) or the endocardium (arrow). Magni®cations £ 40 (A±C), £ 10 (D± G), £ 4 (H±L), £ 5 (M±O).

    Article Snippet: Sections were counterstained with the nuclear stain methyl green (Vector).

    Techniques: Staining, Expressing, Immunohistochemistry, Labeling